Shining too much light on samples can photobleach
them, burning out the fluorescent "bulbs" scientists use to light up cells.
When visualized with PA systems, PAtrace does not photobleach
or degrade at fluences less than 20 mJ/cm2, and generates a stable signal for over 1000 pulses.
A rectangular region was irradiated with high-intensity, 488-nm excitation to photobleach
the green fluorescence while enhancing the red fluorescence of cell membranes there.
(21) The instrument is perimeter-like in design and use (see Figure 2) with the patient required to press a buzzer when they see the test stimulus, which is 2[degrees] in size and has a wavelength of 505nm, after photobleach
. Rather than measuring the full dark adaptation curve, this instrument, in its default setting, uses a reduced photobleach
and presents the stimulus location where rod density may be first reduced in early AMD (around 5[degrees] eccentricity in the superior retina), (8) thus reducing testing time and targeting a retinal area that may be susceptible to functional loss first.
(2002) microinjected QDmicelles into the embryos of the carnivorous frog Xenopus and reported that the QDs were stable, nontoxic, and slow to photobleach
. Further, they suggested QD's lack of interference with embryogenesis (Dubertret et al.
Unlike most fluorochromes used today, these structures do not photobleach
Additionally, quantum dots photobleach
slowly, leading to improved sensitivity.
None of the two used light sources can photobleach
Orange II without a photocatalyst.
Unlike fluorescence and chemiluminescence, resonance light scattering does not photobleach
particles (17, 20-24).
In addition, sensory ablation studies and the use of photobleached
scorpions (Kloock 2009) can be used to investigate the contributions of ocular and extra-ocular (Zwicky 1970a,b) photodctection in mediating these responses.
When the weathering test began, WS/RPP-DB and WS/RPP-WB were photobleached
rapidly, then slightly slowed up to 480 hours.