phytohemagglutinin


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Related to phytohemagglutinin: concanavalin A, phytohemagglutinin test

phytohemagglutinin

[¦fīd·ō‚hē·mə′glüt·ən·ən]
(biochemistry)
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Human PBMCs and SGECs were cocultured at the ratio of 1: 1 for 3 days in the presence of 2.5 [micro]g/ml phytohemagglutinin with or without EVs derived from BM-MSCs or iPSC-MSCs (5 [micro]g/ml) and then examined for gene expressions in isolated PBMCs (a) and SGECs (b) by qRT-PCR.
Nitrotetrazolium blue tetrazolium (NBT), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), phytohemagglutinin (PHA), dioxin, and phosphate-buffered saline (PBS) were obtained from Sigma-Aldrich (St.
CFSE-stained PBMCs (5 x [10.sup.5]; n=6) were cultured in RPMI-1640 containing 10% FCS (Gibco, Invitrogen Corporation), 2 mM L-glutamine, antibiotic-antimycotic cocktail (Sigma-Aldrich Corporation), either with or without phytohemagglutinin (PHA) (1 [micro]g/mL).
Criteria: The positive result: (1) The negative control hole spot points were 0–5, and (A antigen or B antigen spot points) – (the negative control hole spot points) ≥6; (2) while the negative control hole spots ≥6, and (A antigen or antigen B spot points) ≥2 times (the negative control hole spots); the negative result does not meet the positive criteria, and the control hole of aseptic phytohemagglutinin is normal.
Mitogens (Sigma-Aldrich): concanavalin A (Con A) (25 [micro]g/ml), phytohemagglutinin (PHA) (25 [micro]g/ml), pokeweed mitogen (PWM) (2.5 [micro]g/ml), and antigen CD3 (3 [micro]g/ml) (Beckman Coulter) and BWD (0.12 and 75[micro]g/ml) were added in a volume of 25 [micro]l in different exposure intervals (4, 24, 48, and 72h).
Ltd., Saint Louis, Missouri) or 1.2 [micro]g/mL of phytohemagglutinin (PHA; BiochromAG, Berlin), in the presence or absence of two BS extracts (0.1 [micro]g/mL).
Phytohemagglutinin (PHA), which triggers inflammation in macrophages, was used as a positive control.
Phytohemagglutinin (used at doses of 5 [micro]g/mL) and concanavalin A (used at doses of 5 [micro]g/mL) were purchased from Sigma-Aldrich (St.
PBMCs were cultivated without stimulus, stimulated with soluble leishmania antigen (SLA; 5 [micro]g/ml), in the presence or absence of DI4G (300 nM), and phytohemagglutinin (PHA, 10 [micro]l/ml; Gibco BRL, Grand Island, NY) at 37[degrees]C in an atmosphere of 5% C[O.sub.2].
Finally, the most important follow-up testing is that of T cell proliferation in response to mitogens such as phytohemagglutinin (PHA).
Cytogenetic study: Chromosomal analysis was performed after 72 hr phytohemagglutinin (PHA) stimulation of peripheral blood lymphocytes culture based on standard methods.