Inoculation of CVB3 in BALB/c mice resulted in considerably high levels of cardiac viral load (6432 [+ or -] 291 pfu/mg) detected by the plaque assay
on day 4 post-infection (Fig.
Aliquots of these samples were also cultured as described above to determine the cell count, and plaque assays
were conducted to determine the concentration of phage.
Moreover, the gelatin-based medium could be directly dissolved for further quantification by plaque assay
and without the elution procedure.
No pools produced live virus isolates in the plaque assay
Virus quantification was performed in 24-well tissue culture plates, titrated on the basis of plaque forming units (PFU) count by plaque assay
(Burleson et al.
However, when the plaque assay
was used, the culture process lasted almost 3 d because of the slow bacterial growth rate.
After 48 h at 37[degrees]C, infected cells were harvested with 3 freeze-and-thaw cycles, and cellular debris were removed with low-speed centrifugation, and virus titer was measured by standard plaque assay
(Killington and Powell, 1991).
015 plaque-forming units/mL in negative human plasma and subjected it to the plaque assay
and real-time PCR.
Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay
The virus was grown and titrated in Vero cells by plaque assay
by means of Vero cells overlaid with 1% low melting agar in usual growth media.
albopictus mosquitoes that were sent to CDC in December 1991; 9350 were tested, in 96 pools, for virus isolation by plaque assay
in Vero cell culture.
In addition, day 0 control mosquitoes that had been stored at -80[degrees]C and all mosquitoes collected on days 2-30 were assayed for viable virus by using Vero plaque assay
cell culture as described (3).