PBMCs were cultured with phytohemagglutinin (PHA; graphics (a) and (c)) or pokeweed mitogens
(PWM; graphics (b) and (d)) and stained with carboxyfluorescein diacetate succinimidyl ester cell tracer (CFSE).
100 [micro]L 900 [micro]L PWM: Pokeweed mitogen
, ConA: Concanavalin A, HSP70: Heat Shock Protein 70, A.a.: Aggregatibacter actinomycetemcomitans, P.g.: Porphyromonas gingivalis, T.f.: Tannerellaforsythia, and unstim.: without stimulation.
Proliferation of chicken blood leukocytes in pokeweed mitogen
is macrophage dependent.
The culture medium contained 5 [micro]g/ml concanavalin A (Con A), 10 [micro]g/ml phytohemagglutinin (PHA), or 5 [micro]g/ml pokeweed mitogen
(PWM) to determine the lymphocyte response to mitogen stimulation.
The cells were incubated for 96h with both VA-E at 0, 10, 100 and 1000 [micro]g/ml, interleukin-6 at 1000 U/ml, and pokeweed mitogen
and phytohaemagglutinine at 2.5 [micro]g/ml.