The nanoparticle density was adjusted to 1.21 g/mL with KBr and 6 mL of LDE-paclitaxel were placed on 12.5 cm ultracentrifugation tubes (polyallomer
tubes, Beckman, USA).
Briefly, lymphocytes were lysed in polyallomer
centrifuge tubes (14 ram; Beckman, Fullerton, CA, USA) by addition of 3.1 mL lysis solution (0.25 M [Na.sub.2]EDTA, 2% wt/vol sarcosyl, 10 mM Tris-base, pH 7.4) to 0.1 mL cell suspension.
Plasma (3 ml) was adjusted to a density of 1.41 g/ml with KBr in Beckmann Polyallomer
Centrifuge tubes (No.
The supernatant from this step was transferred to polyallomer
centrifuge tubes, diluted with homogenization buffer to 500 [mu]1, and centrifuged for 90 min at 100,000 X g using a Beckman Optima L-90K ultracentrifuge and SW65 rotor.
In a browse through some supply catalogues, l spotted the polyallomer
1.5-mL microcentrifuge tube.
HDPE (1956), PC (1957), PP (1958), phenoxy (1962), polyallomer
(1962), ionomer (1964), polypropylene oxide (PPO) (1964), ethylene-vinylacetate (1964).
Method 5-mL 1-mL Ultracentrifugation Rotor Type 50.3 Ti Type 25 Tube Polyallomer
bell top Polycarbonate quick seal (cat.
The density-adjusted plasma (~ 4 mL) was layered below 7 mL of a KBr solution (density = 1.005 kg/L), pH ~7.4, containing 1 g/L disodium EDTA in 11-mL Optiseal polyallomer
The density-adjusted plasma (3.5 mL) was layered below EDTA containing a 1 g/L KBr solution (density = 1.006; pH 7.4) in Optiseal polyallomer
tubes (Beckman) and centrifuged for 2.5 h under reduced pressure in a Beckman XL-70 centrifuge at 30OOO g rpm at 7[degrees]C using a NVT65 rotor.
Isopycnic ultracentrifugation was performed on 3-4 mL of plasma by standard methods using KBr at a solvent density of 1.21 kg/L in 6-mL polyallomer
Quick Seal tubes at 280 000g for 48 h at 4[degrees]C in a 40.3 Ti rotor (Beckman Instruments).
The plasma (3 mL) was deposited in a centrifuge tube (Kontron polyallomer
tubes, total volume, of 13 mL; Kontron) containing 0.1155 g of KBr (Sigma).
After a two-layer gradient was made in a 5.1-mL Quick-Seal polyallomer
tube (Beckman Instruments) by layering normal saline containing 100 mg/L of [Na.sub.2]EDTA on 1.5 mL of density-adjusted plasma, a single spin gradient ultracentrifugation at 228 000g at 4 [degrees]C for 90 min (VTi 65.2 rotor, Beckman Instruments) was performed to isolate LDL.