of the mouse obese gene and its human homologue.
However, the importance of spontaneous diabetes models has been relatively underestimated owing to the difficulty of positional cloning
Collectively, the application of these methods to the isolation of human disease genes was known as "positional cloning
," and the early 1990s saw the emergence of several biotechnology companies founded to capitalize on the confluence of these technologies.
Boyce Thompson scientists conducted positional cloning
and determined that the uniform ripening gene was located at a specific area on chromosome 10.
Subjects addressed include: the rat as a model in biomedical research, genetic mapping and positional cloning
, procedures for somatic cell transfer, the use of lentiviral vectors to obtain transgenic rats, generation of gene-specific mutated rats using zing-finger nucleases, and rat genomics as applied to psychiatric research.
The gene responsible for the trait is then identified through positional cloning
(8,9) Lin et al., (10) in a study using positional cloning
in a well-established canine model, found that canine narcolepsy is caused by disruption of the hypocretin (orexin) receptor 2 genes (Hcrtr2).
of the mouse obese gene and its human homologue." Nature, 1994; 372(6505):425-32.
of the wheat vernalization gene VRNI.
GEFS+ was identified with the use of positional cloning
, which is currently the most useful genetic technique to identify families with many affected individuals, he noted.
GEFS+ was identified using positional cloning
, which is currently the most useful genetic technique to identify families with many affected individuals, he said.