primary culture


Also found in: Dictionary, Thesaurus, Medical, Legal, Financial.
Related to primary culture: secondary culture

primary culture

[¦prī‚mer·ē ′kəl·chər]
(biology)
A tissue culture started from cells, tissues, or organs taken directly from the organism.
Mentioned in ?
References in periodicals archive ?
It was concluded that if this primary culture is supported with growth stimulators, growth rate can be more accelerated and the culture can be used in several types of research fields such as production of recombinant proteins.
We used endometrial cells in primary culture to detect the response of TGF-[sz]1 and HIF-1a to VEGF, and we found that HIF-1a could regulate VEGF expression directly.
It is important to note that some of these cells constitute cell lines (RF/6a) while others constitute primary cultures (BRECs, HRECs, and HUVECs).
In this study, 24 h after the primary culture, some cells were attached to the flasks and morphology of cells was changed to fibroblast-like shape.
We verified the identification of human preadipocytes from the primary culture of orbital adipose tissue by staining for Pref-1, a marker expressed preferentially in preadipocytes, rather than mature adipocytes.
The isolated trophoblastic cells showed faster growth pattern in primary culture on PGCs, in one-week colony diameter measured 7.0 [+ or -] 0.3 mm, compared to those cultured on MEFs; colonies grew to 5.7 [+ or -] 0.4 mm (P < 0.05; Figures 2(a) and 2(b)), whereas, on subsequent cultures (2nd-10th) the difference in colony morphology, although it was visually larger when trophoblasts were cultured on PGCs compared to MEFs, did not reach statistical significance (P > 0.05;Figures 2(c), 2(d), 2(e), and 2(f)).
Ex vivo model was developed on the basis of primary culture deriving from normal (unaffected by atherosclerosis) human aortic intima (Orekhov 1990).
Effect of some phthalate ea-ters and other testicular toxins on primary cultures of testicular cells.
After the primary culture, when adherent cells reached to 90% confluency, they were detached with 0.25% trypsin-EDTA solution.
Difficulty during the primary culture establishment is also frequently encountered.

Full browser ?