Caption: Figure 1--Phalaenopsis Classic Spotted Pink hybrid development stages (at 120 DASI), in somatic embryos until complete plant: (A) Protocorm showing the formation of morphogenic structures at the explant base, 15 days after SE-induction; (B) Protocorm showing leaf
primordia etiolation (#) and morphogenic structure formation at the base (arrow) (20x); (C) Protocorm showing embryos in different stages at the 30th culture day: globular (*), Torpedo (#) and protocormoids ([section]); (D and E) Chlorophyllated protocormoids from somatic embryos; (F) Complete plants derived from somatic embryos generated from protocorms.
The ancestral state of the family, is reconstructed as having a hemispherically shaped scutellum and undifferentiated leaf
primordia, similar to those found in Juncaceae and some Mapanioideae.
The premuscular
primordia appear more positive than the undifferentiated mesenchyme.
The stomodeum is also clearly visible, and there is an initial differentiation of the mandibules, maxillules, maxillae, and maxilliped
primordia. The telson reaches the level of the maxillae (Fig.
Snow, "The dorsiventrality of leaf
primordia," New Phytologist, vol.
It has been reported that Pax-2 is one of the earliest markers of the developing inner ear, which was expressed in the auditory and vestibular sensory
primordia [26, 27] and related to the highly proliferative potential of progenitor cells in developing sensory patches of chicken [28].
Among all these tested combinations, shoot
primordia appeared on the calli on SIM1and SIM2 media only after 28 days of sub-culturing(Fig.
tuoliensis is the earliest variety from Xinjiang in China; it needs low temperatures for bud stage and temperature <4degC for the differentiation of mushroom
primordia, which are the bottleneck of P.
The emergence of root
primordia in air layers, occurred with a continuous vertical linear pattern above the cut area, extending towards the distal region of the branch, which may indicate an origin from ray cells.
In wandering third stadium larvae, pb-GAL4 is expressed in a small ring of cells in the aristal
primordia, which was observed in the early prepupae (Figure 2(F)).
On shoot apex longitudinal section was possible to observe standard type colleters between leaf
primordia and stipule, emerge on secretion (fig.
Further, during flowering the formation of pistillate organs may be favoured by high auxin level in the vicinity of differentiating
primordia.