At the same time, these proteins recruit key molecules that are responsible for mediating the intracellular signal, among these, procaspase
8 and subsequent formation of the DISC complex [13-15, 35].
Primary antibodies for procaspase
3, caspase 8, caspase 9, CDK6, p53, and cleaved PARP were purchased from Cell Signaling Technology (Beverly, MA).
Abundant expression of Dec1/stra13/sharp2 in colon carcinoma: its antagonizing role in serum deprivation-induced apoptosis and selective inhibition of procaspase
Cleavage of inactive procaspases
to active caspase enzymes is a hallmark of apoptosis.
IFN can also induce the synthesis of gene products that arrest the cell cycle such as p21 and thus block virus replication, or induce a pro-apoptotic state such as procaspases
. IFN can induce the synthesis of proteins that are involved in the processing and presentation of virus proteins to [CD8.sup.+] cytotoxic T lymphocytes.
The mitochondria is the central regulator of the intrinsic pathway of apoptosis and that is accompanied by the release of procaspases
, cytochrome c, apoptotic protease-activating factor 1 (Apaf-1), endonuclease G and apoptosis-inducing factor (Green 2000).