prorennin

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prorennin

[prō′ren·ən]
(biochemistry)
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
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The PCR production was then cloned into the pMD19-T vector (TAKARA, Dalian, China), denominated as pMD19-TPreprochy, and the inserted gene fragment was sequenced by Sangon Biotech Co., Ltd (Shanghai, China).The preprochymosin, prochymosin, and chymosin genes were amplified from pMD19-T-Preprochy using gene specific primers: Pre-F and Chy-R, Pro-F and Chy-R, Chy-F and Chy-R (Table 1), and then the amplified fragments were inserted into pMD19-T vector, denominated as pMD19-T-Pre, pMD19-T-Pro, and pMD19-T-Chy respectively.
Three XhoI and XbaI fragments of preprochymosin, prochymosin, and chymosin were obtained from the pMD19-T vector containing the same cDNA sequences and were cloned into the pPICZ[alpha]A vector previously digested with the same restriction enzyme.
Isolation and cloning of yak preprochymosin, prochymosin and chymosin genes
Using RT-PCR and the gene-specific primer pairs Pre-F and Chy-R, Pro-F and Chy-R, and Chy-F and Chy-R (Table 1), three bands of sizes 1,182, 1,135, and 1,008 bp were obtained (Figure 1) corresponding to the preprochymosin, prochymosin, and chymosin genes, respectively.
The Yak chymosin gene has a 1,146 bp open reading frame and encodes 381 amino acids which also contains a 16-amino acid signal peptide that is removed to generate prochymosin from preprochymosin.
Rennet can be divided into three types: prochymosin A, B, and C.