The Company's novel proenzyme
therapy is based on the science that enzymes stimulate biological reactions in the body, especially enzymes secreted by the pancreas.
According to the company, the data provides the strongest evidence that proenzymes
could be an effective tool in the fight against metastatic cancer, the single biggest cause of patient death for sufferers.
Metalloproteinases inactivation is based on forming complexes which consist of active forms of MMP or some proenzymes
and tissue inhibitor, complexes MMP-TIMP [35, 36].
Caspases are the 30-60 KDa proenzymes
Thus MMPs are regulated in several ways: during gene expression, by the need for activation of latent proenzymes
and by being bound to inhibitory molecules.
There are two distinct gelatinases of the MMPs family, MMP-2 and MMP-9, which are also found as proenzymes
, produced by basal cells for physiologic control of hoof growth (FRENCH & POLLITT, 2004).
were activated immediately prior to use with p-aminophenylmercuric acetate (APMA 2mM for 1h at 37[degrees] C for MMP-2 and MMP-8, APMA 2mM for 2h at 37[degrees]C for MMP-1, and APMA 1mM for 1h at 37[degrees]C for MMP-9).
MASP1, MASP-2, and MASP-3 are synthesised as proenzymes
with apparent molecular weights of 90 kDa, 74 kDa, and 94 kDa, respectively.
The minerals are required to turn inert proenzymes
into active biological catalysts.
MMPs tend to be secreted as proenzymes
that require activation to reveal their enzyme activity, and the action of many of them is also controlled by their interactions with other proteins called 'TIMPs'--tissue inhibitors of metalloproteinases.
Caspases are present as proenzymes
that are cleaved and activated during apoptosis, of which caspase-3 acts as an apoptotic executor.
They are then cleaved upon release from the secretory pathway to become proenzymes