The erythropoiesis in bone marrow was lowered by inflammatory cytokines, the process of which was mainly resulted from the blockage of erythroid progenitor cell proliferation and proerythroblast
It is obvious that, unlike the erythrocyte, proerythroblast
has a ripple profilogram in the central region, due to the presence of heterodispersed polymorphic granules.
Consequently, it is critical for iron to be available to the developing erythrocyte during the 3day window in which the proerythroblast
develops into the reticulocyte.
The first RBC precursor, called the "proerythroblast
," divides, develops, and eventually becomes filled with hemoglobin.
Erythroid differentiation started with the expression of CD71 (22.8% [CD71.sup.+]/[Ter119.sup.-] KO versus 27.9% [CD71.sup.+]/[Ter119.sup.-] NTC cells) in the proerythroblasts
. The maturation into early erythroblast cells was indicated by [CD71.sup.high]/[Ter119.sup.-] expression (1.4% KO versus 2.7% NTC), and late erythroblasts cells expressed [CD71.sup.high]/[Ter119.sup.+] (2.3% KO versus 4.9% NTC).
Supplementary Figure 1: Representative cytospin images of proerythroblasts
(Pro-E), basophilic erythroblasts (Baso-E), polychromatic erythroblasts (Poly-E), orthochromatic erythroblasts (Ortho-E), and mature erythrocytes.