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They were found in proestrus, estrus or metestrus and were considered as reproductive females since there is hormonal stimulation for each phase and a clear indication that they are sexually receptive.
The females were paired and naturally mated on days 11, 14 and 16 from the initial day of proestrus.
Normally, the estrus cycle in rats lasts 4-5 days (16), but in the present study a high level of progesterone concentration in LDE and HDE groups enhanced the duration of estrous cycles due to longer duration of diestrus phase and shorter duration of estrus and proestrus phases in these groups.
Male and regularly cycling females in proestrus and estrus were decapitated after 0 minutes (control group, the N group, without any injection) 5, 15, 30 and 60 min.
in the evening of proestrus and collected into a heparinised bottle.
The rotarod test did not denoted effects of drug on motor coordination of rats during the phases of proestrus and diestrus (figure not shown).
For mating purposes, the phase of the estral cycle of each female rat was observed in the morning by means of a vaginal smear, classifying the phase as proestrus (12 h), estrus (14h.
A proestrus smear consists of a predominance of nucleated epithelial cells; an estrus smear primarily shows anucleated cornified cells; a metaestrus smear contains the same proportion among leukocytes, cornified, and nucleated epithelial cells; and a diestrus smear primarily consists of a predominance of leukocytes.
Females in proestrus stage were caged overnight with males of the same stock.
Females during proestrus cycle were kept near males.
However, two peaks periods of 17[beta]-estradiol were observed for all samples, the first in the metestrus and the second during the proestrus (Fig.
La presencia de celulas nucleadas cornificadas y epiteliales en el moco vaginal dio evidencia de la condicion de proestrus o estrus del ciclo estral .
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