In the traditional study, the protoplast
regeneration applied in genome shuffling was carried out on agar plate.
Nutritional requirements for growth of Vicia hajastana cells and protoplasts
at a very low population density in liquid media.
giganteus) by protoplast
fusion using polyethylene glycol (PEG).
The transcriptional activity of CMYB1 was examined via dual luciferase reporter assay system in rice protoplast
were transformed with effector plasmids and reporter plasmid containing GAL4, DRE fragments, and minimal cauliflower mosaic virus (CaMV) 35S promoter in upstream of luciferase.
Cell suspensions are also suitable for protoplast
isolation in studies of transitory genetic expression and transformation by Agrobacterium (Torabi et al.
harzianum strains, Th12A.10.1 (UV irradiation of Th12) and ThF2-1 (protoplast
fusion, ThV x Th291) (Besoain et al., 2007) and the corresponding parental strains were used.
For mass propagation, mutation induced breeding, somatic embryo genesis, protoplast
culture, and cell suspension culture, callus induction is the most vital phase among the in vitro studies on banana.
On the basis of experiment with single, non-statolith containing cells, we developed a new model for plant gravity sensing in which we suggest that the entire protoplast
acts as the gravity sensor.
have used EMS mutagenesis and interspecific protoplast
fusion to isolate improved probiotic yeasts.
Establishment of embryogenic callus and high protoplast
yielding suspension cultures of sugarcane (Saccharum spp.
Among specific topics are the protoplast
transformation of filamentous fungi, developing Impala-based transposon systems for tagging genes in filamentous fungi, identifying differently expressed fungal genes in planta by suppression subtraction hybridization, applying laser micro-dissection to study plant-fungal pathogen interactions, measuring protein kinase and sugar kinase activity in plant pathogenic Fusarium species, and evaluating anti-fungal susceptibility using flow cytometry.
The two methods used in the lab are protoplast
formation and spore germination.