protoplast fusion


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protoplast fusion

[′prōd·ə‚plast ‚fyü·zhən]
(genetics)
A technique by which two protoplasts are fused to form hybrid cells that can grow into mature hybrid organisms; usually performed on plants.
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increments; Capacitance for 10 500V, 25 3,275 oF; 500 2,500V, 25 and 50 oF; LCD; supply with 1 and 2 mm gap disposable cuvettes (50 each), Chamber for plant protoplast fusion (one), Electrodes (2mm gap) covering ~90mm petri-dish for plant applications (one), micro-slides for cell/protoplast fusion 1mm gap (10 numbers); with suitable automatic voltage stabilizer.
Somatic hybridization by protoplast fusion is a promising technique for breeding ornamental species and requires reliable in vitro protocols.
Protoplast diameter is information that can be used in hybridization studies using electriofusion so that an inverse relationship exists between protoplast diameter and the voltage necessary to promote protoplast fusion.
giganteus) by protoplast fusion using polyethylene glycol (PEG).
Production of fertile somatic hybrid plants of sunflower and Helianthus giganteus L by protoplast fusion.
Additional Maxygen patents pending in the United States, Europe and other countries specifically cover protoplast fusion methods of whole-genome recombination.
Sreenath and his team hope that, once they have it, they will be able to use protoplast fusion to induce the gene fragments of fuse with a more commercially viable cultivar and create the first caffeine-free Arabica or Robusta.
Using a mix of conventional and advanced breeding techniques -- such as anther culture, tissue culture, hybrid sorting, and protoplast fusion -- B&W eventually produced Y-1.
The team is relying on a technique called protoplast fusion to move the insect-resistance trait, explains Eash.
Other wild species are so genetically different that they can't be crossed with cultivated potatoes, so Brown and colleague Sandra Austin-Phillips, at the University of Wisconsin, are using protoplast fusion to make hybrids of the two.
Austin-Phillips has already produced a potato plant by protoplast fusion that can be used in breeding nematode resistance.
Their current approach -- protoplast fusion -- merges the contents of a cell from each selected genus, and then regenerates the hybrid.