Intra and Inter-specific protoplast fusion
was carried out by the method given by Prabavathy et al.
These lines can be further utilized for protoplast fusion
, genetic transformation and as a tool for basic studies of plant biochemistry and molecular biology.
Thus, compared to the traditional protoplast fusion
techniques, our genome shuffling method has the advantages of high efficiency and easy operation.
harzianum strains, Th12A.10.1 (UV irradiation of Th12) and ThF2-1 (protoplast fusion
, ThV x Th291) (Besoain et al., 2007) and the corresponding parental strains were used.
Genetic construction of potentially probiotic Saccharomyces boulardii yeast strains using intraspecific protoplast fusion
. J Appl Sci 2007;3(3):209-217.
Protoplast diameter is information that can be used in hybridization studies using electriofusion so that an inverse relationship exists between protoplast diameter and the voltage necessary to promote protoplast fusion
Production of fertile somatic hybrid plants of sunflower and Helianthus giganteus L by protoplast fusion
. Plant Cell Rep., 14: 232-235.
Keywords: Solanum, protoplast fusion
, polyethylene glycol, somatic hybrid, RAPD analysis.
It began some 15 years ago, when Gary Harman, a Cornell professor of horticultural science, and Tom Stasz, then a research associate, took two different strains of trichoderma that occur naturally in soil and, using protoplast fusion
, bioengineered a new strain.
Using a mix of conventional and advanced breeding techniques -- such as anther culture, tissue culture, hybrid sorting, and protoplast fusion
-- B&W eventually produced Y-1.
By using tissue cultures, breeders have used protoplast fusion
as a non-sexual means of introducing new genetic material into specific plants.
The team is relying on a technique called protoplast fusion
to move the insect-resistance trait, explains Eash.