METHODS: cry4, a mosquitocidal protoxin gene, was amplified by E-PCR with family and type primers that were designed from known conserved and unique regions, respectively.
thuringiensis for novel protoxin genes is to have a method that can rapidly and accurately characterize the novel subclass genes.
To test the efficiency and specificity of E-PCR with DGGE approach, we used a mosquitocidal protoxin class gene, cry4, in Bacillus thuringiensis as subject.
israelensis was used as a positive control strain, because of its well studied protoxin genes.