Our ability to place the tissue easily in the tissue holder slot, scan quickly with seamless stitching of the images within a few minutes, and acquire high-resolution images with instant pseudocoloring
enabled us to achieve excellent results in the current study.
Ogunbona, "ConvNets-based action recognition from depth maps through virtual cameras and Pseudocoloring
," in Proceedings of the 23rd ACM International Conference on Multimedia, pp.
and other image analyses were performed using AxioVision Release 4.6 software.
The intensity of autofluorescence, green (550 nm) and red (610 nm) reflectance images, was provided for pseudocoloring
. An image processor makes autofluorescence images to green color, the green reflectance image to red color, and the red reflectance image to blue color, then AFI pseudocolored images were composited.
Color processing includes color space transformations, pseudocoloring
, and hue and saturation adjustments.
was performed by inverting the matrix conversion process presented by Ruifrok and Johnston.
and overlaying of the emission channels were performed with Adobe Photoshop (Version 7.0, Adobe, San Jose, California); however, no other manipulation of the images was involved.