However, there were significant effects of light regime, temperature, substrate and their interactions, including a triple interaction, on pycnidiospore production (Table 1).
Stenocarpella maydis responded differently to the qualitative factors of substrate and light regime for pycnidiospore production and percentage of germination (Table 2).
1] and continuous light regimes on pycnidiospore production and the percentage of germination (Table 2).
The adjusted polynomial regression of all substrates for pycnidiospore production and percentage of germination were significant (p = 0.
maydis pycnidiospore production (Figure 1) and percentage of germination (Figure 2) for all substrates tested.
maydis pycnidiospore germination percentages of 86% and 96% on an oak grain substrate and mineral salt medium, respectively.
1] after 14 days of incubation, which is slightly more than half of the number produced using Ullstrup's (1970) method; however, these spores numbers were achieved in a significantly shorter time, demonstrating the efficiency of the present method to produce viable pycnidiospore inocula for experimental research.
Analysis of covariance (ANCOVA) of pycnidiospore production [g.
Previous research has shown that the viability of pycnidiospores decreases with continued exposure to sunlight (BRUNELLI et al.
1] cycle yielded the largest number of pycnidiospores per gram (67,600), 93% of which germinated after 14 days of incubation; therefore, these conditions are recommended for mass inoculum production (Figures 1 and 2).