Chromatolysis and pycnotic
changes, as well as a decrease (and sometimes a complete absence) of neuroendocrine granules, were dominated.
Testes of animals in Group 2 and 3 exhibited disordered, sloughed germinal cells with shrunken pycnotic
nuclei and less distinct seminiferous tubule borders.
Cr (VI) induced necrosis in tubular epithelium, pycnotic
nuclei in the hematopoietic tissue, narrowing of the tubular lumen and expansion of spaces inside the Bowman's capsule (Ahmad et al., 2006; Velmurugan et al., 2007; Ashish and Banalata, 2008; Pedro and Alicia, 2008; Lushchak et al., 2008; Mishra and Mohanty, 2009; Sannadurgappa and Aladakatti, 2010).
Apoptotic cells were identified by their pycnotic
nuclei, which label strongly with acridine orange, as previously described (Foster and McFall-Ngai, 1998).
Apoptosis was determined by scoring cells displaying pycnotic
nuclei (visualized with Hoechst 33342) .
tumour cells or ruptured blood vessels were found at 24 h after 0.5 h-delayed PDT.
It is possible to observe a thin corneal layer of flattened cells and pycnotic
4a), which was characterized by necrotic hemocytes and pycnotic
cells (see Fig.
Diffuse ciliar desquamation of epithelial cells accompanied by areas of degeneration was observed; some cells both were pycnotic
and had commenced apoptosis.