This drug also acts on the mevalonate pathway and inhibits farnesyl pyrophosphatase
, which is the key enzyme in cholesterol synthesis.
Genetic improvement of sugarcane for drought and salinity stress tolerance using Arabidopsis vacuolar pyrophosphatase
Since the 3D structure of the ectonucleotide pyrophosphatase
is yet to be revealed, the mutation was not modeled.
Sustained osteomalacia of long bones despite major improvement in other hypophosphatasia-related mineral deficits in tissue nonspecific alkaline phosphatase/ nucleotide pyrophosphatase
phosphodiesterase 1 doubledeficient mice.
In addition, molecular dynamics simulations predicted that a loop of the core pyrophosphatase
containing F82 and L83 acts like a wedge to open the capping helices and that mutation of these residues to Gly would result in the collapse of the capping domain into a fully closed state (Figure 7(a)) .
ppaC encodes inorganic pyrophosphatase
(PPase) with the DHHA2 domain (IPR004097) and pyrophosphatase
activity (GO: 0016462).
Molland et al., "Synthesis of 3-(3aryl-pyrrolidin-1-yl)-5-aryl-1,2,4-triazines that have antibacterial activity and also inhibit inorganic pyrophosphatase
," Bioorganic & Medicinal Chemistry, vol.
Based on a [Cu.sup.2+]/GQ DNAzyme, an electrochemical method for pyrophosphatase
(PPase) activity detection was developed .
CD38 converts [NAD.sup.+] to ADPR, which in turn is metabolized to AMP by CD203a/PC-1 (an ectonucleotide pyrophosphatase
ADP-ribosyl cyclase, 8-Br-cADPR, alcohol dehydrogenase, resazurin, NAD, nicotinamide, bovine serum albumin (BSA), diaphorase, 3-[4,5-dimethylthiazol-2-yl]- 2,5-diphenyltetrazolium bromide (MTT), bicine, alkaline phosphatase, riboflavin 5'-mono-phosphate (FMN), NADase, nucleotide pyrophosphatase
, and ethylene diamine tetraacetic acid (EDTA)-Na were purchased from Sigma (St.
(31) For in vitro ubiquitination assay, reactions were carried out using a ubiquitinylation kit purchased from Enzo Life Sciences (Farmingdale, NY, USA) containing 2.5 [micro]M biotinylated or non-tagged ubiquitin, 100 nM His-tagged El, approximately 1-2.5 [micro]M His-tagged E2, and 5mM Mg-ATP, and 20U/mL yeast inorganic pyrophosphatase
(New England Biolabs, Ipswich, Massachusetts, USA).
It is believed that this sequestration of sodium ions is attained by tonoplast Na+/H+ antiporters (Flowers and Colmer, 2008) and pyrophosphatase
(Guo et al., 2006; Krebs et al., 2010).