(PK) deficiency is a rare, inherited disease that presents as chronic hemolytic anemia, which is the accelerated destruction of red blood cells.
(PK) is one of important rate-limiting enzymes involved in glycolysis (Yuan et al., 2013).
Veldthuis et al., "Partial pyruvate kinase
deficiency aggravates the phenotypic expression of band 3 deficiency in a family with hereditary spherocytosis," American Journal of Hematology, vol.
Schuman et al., "Identification of small molecule inhibitors of pyruvate kinase
M2," Biochemical Pharmacology, vol.
Lepper TW, Oliveira E, Koch GDW, Berlese DB, Feksa LR (2010) Lead inhibits in vitro creatine kinase and pyruvate kinase
activity in brain cortex of rats.
Would occur for concentrations 10 [micro]g/L and 20 [micro]g/L excess of substrate (glucose) for the enzyme pyruvate kinase
(which degrades glucose to pyruvate to borrow the Krebs cycle and the process of breathing with the formation energy ATP), may be this is due to saturation of receptors and therefore, part of the glucose escape hepatocytes experimentation by the mechanism of facilitated transport of glucose across the biological membrane to its action on PEPCK level explained above kernel this would explain the values of the variation of the high optical density of the extracts at concentrations 10 [micro]g/L and 20 [micro]g/L in contrast to those obtained for concentrations 2 [micro]g/L and 5 [micro]g/L.
Fructose-1,6-bisphosphatase activity was measured by the method of Gancedo and Gancedo (1971), pyruvate kinase
activity was estimated by the method of Pogson and Denton (1967), lactate dehydrogenase activity was estimated by the method of King (1965), glycogen synthase activity was estimated by the method of Leloir and Goldemberg (1962), glycogen phosphorylase activity was estimated by the method of Cornblath et al.
Figure 2 showed that enzymes related to glycolysis (fructose-bisphosphate aldolase (GI: 27469074), glyceraldehyde 3-phosphate dehydrogenase (GI: 27467475), enolase (GI: 27467479), pyruvate kinase
(GI: 27468291)), the TCA cycle (succinyl-CoA synthase (GI: 27467842), and isocitrate dehydrogenase (GI: 27468288)) were downregulated at the post-exponential phase.
The enzymes particularly upregulated were hexokinase, aldolase, enolase, pyruvate kinase
, and adenosine deaminase .
Oxidative stress drives activated FOXO1 to enter the cell nucleus, suppressing expression of genes involved in glucose metabolism, such as glucose transporter-2 (GLUT2) and pyruvate kinase
Previous studies on some of the key glycolytic enzymes (glycogen phosphorylase, glycogen synthase, pyruvate kinase
, and pyruvate dehydrogenase) within the foot muscle of L.
Rathmell, "New roles for pyruvate kinase
M2: working out the Warburg effect," Trends in Biochemical Sciences, vol.