For each strain transferred to our laboratory, we promptly identified capsular type by quellung
reaction, and resistance genotype was determined by real-time PCR.
Results were confirmed by the Quellung
reaction with newly available antiserum to identify 6C serotype (Statens Serum Institute, Copenhagen, Denmark).
Serotype 6C was identified in 2007 on the basis of its distinct binding patterns with 2 monoclonal antibodies; serotype 6C had previously been typed as 6A according to the standard quellung
Isolates were serotyped by using the Quellung
pneumoniae by standard methods, and serotypes were determined by the Quellung
Conventional serotyping using the Quellung
method was performed where possible; culture-negative or incompletely genotyped PFs were serotyped by using a real-time PCR that targets different capsular locus genes (13).