Serotyping based on the
Quellung reaction requires expensive reagents and substantial training and experience to perform reliably.
and Hausdorf, A.: 1942, Kristalstruktur und innerkristalline
Quellung der Montmorillonits, Z.
All pneumococcal isolates that showed a positive
Quellung reaction with commercial type-specific antisera (Statens Serum Institute, Copenhagen, Denmark) were assigned a serotype designation (11).
Las tecnicas para determinar el serotipo capsular se basaron en la reaccion de
Quellung, con el uso de antisueros procedentes de Statens Serum Institut, de Dinamarca (25, 26).
Pneumococcal isolates were sent to reference laboratories for serotyping by the
quellung reaction and were categorized as vaccine-type (VT) (serotypes included in PCV7) or nonvaccine-type (NT) (all other serotypes).
Typing antisera factors 29b and 35c are the CDC
Quellung reagents definitive for serotype 35B.
Serotyping was performed by use of the Neufeld
Quellung reaction, a technique previously described (22) by using polyclonal antisera.
Thirty strains were sent to CDC for serotyping but could not be typed using the
Quellung reaction.
Pneumococci were serotyped by the
Quellung method (Statens Serum Institut, Copenhagen, Denmark).
All pneumococci isolated in 1998 and 1999 were serotyped using the
quellung reaction.
Serotypes were determined by the capsular
quellung reaction by using antiserum purchased from the Statens Serum Institute (Copenhagen, Denmark).
Isolates received at the Spanish Reference Laboratory for Pneumococci were serotyped by using the
quellung reaction and antisera provided by the Staten Serum Institute (Copenhagen, Denmark) (8).
