Serotyping based on the Quellung reaction
requires expensive reagents and substantial training and experience to perform reliably.
Serotyping was performed by using the quellung reaction
with antiserum for serogroup 6, factor 6b, and factor 6c (Statens Serum Institute, Copenhagen, Denmark).
In 51 of these, a pneumococcal serotype could be identified via the conventional Quellung reaction
. Evidence of pneumococcal infection in 99 (84%) of 118 culture-negative PF samples was found on the basis of ply or wzg gene detection.
Serotyping, performed by Quellung reaction
, showed a 35F serotype.
All pneumococcal isolates were serotyped according to standard methods by using the Quellung reaction
Serotyping was conducted by using the quellung reaction
with reagents from Statens Serum Institut (Copenhagen, Denmark) (18).