Measurements of biochemical parameters: Ten mL of fasting blood was obtained within 24 hours of the admission and analyzed for serum folate, homocysteine, glucose, total cholesterol, low density lipoprotein (LDL)-cholesterol, high density lipoprotein (HDL)-cholesterol, triglycerides and ferritin using commercially available kit methods (Roche Diagnostics, USA), while serum vitamin B12 was assayed using a radioassay
Mosbach, Molecularly imprinted monodisperse microspheres for competitive radioassay
Direct detection of UDP with a fluorescence polarization (FP) readout provides a safe, HTS-compatible alternative to cumbersome radioassay
methods and is more sensitive and less subject to interference than coupled assay methods, where the UDP is converted to another product.
Changes in measurement procedure from a radioassay
to a microbiologic assay necessitate adjustment of serum and RBC folate concentrations in the U.
For example, folate concentrations were largely undetectable with the metabolomics platform but were measurable in NHS samples using a radioassay
Vitamin B12 and folate were measured using the SimulTRAC-SNB Radioassay
Kit (DRG Instruments GmbH, Germany).
Competitive protein-binding radioassay
FLUOR[tm] 15, purchased from Packard) was added prior to radioassay
using a Liquid Scintillation Counter (TriCarb Model TR 2550 AB).
Vitamins in food and urine samples were determined microbiologically and plasma pyridoxal phosphate by a tyrosine apodecarboxylase radioassay
Measurements by competitive binding radioassay
of serum anti-microsomal and anti-thyroglobulin antibodies in Graves' disease and other thyroid disorders.
The primary method for measurement of serum vitamin B12 is microbiological or, more usually, radioassay
In: GE Abraham's Radioassay
Systems in Clinical Endocrinology.