Radiochromatograms were carried out with a Raytest miniGITA-Star [gamma] Radiochromatography
(Raytest, Straubenhardt, Germany) or with a Bioscan AR-2000 radio-TLC Imaging Scanner (Bioscan Inc., Washington, DC).
The radiochemical purity is estimated by paper, TLC, or gel radiochromatography
. The stability can be also checked by incubation in saline and serum.
The authors also mentioned that losses of radiolabeled selenium (up to 33%) occurred during the radiochromatography
, which they thought indicated volatile selenium constituents in the urine.