DISCUSSION--Genetic Variability Within Subpopulations--A lack of significant differences between observed heterozygosity and expected heterozygosity suggests random mating
and nonsignificant levels of inbreeding in all three subpopulations.
[F.sub.is] which measures the deviation from random mating
, was negative and close to zero implying a small amount of mating between subpopulations, which helped to minimize inbreeding.
Second, the mating structure to be analyzed, rather than being a simple [F.sub.2] or backcross, comprised ten generations of random mating
of the population cross followed by two generations of selfing.
Hardy-Weinberg testing of 10 to 11 polymophic loci in each population revealed no significant deviations from random mating
expectations after application of a sequential Bonferroni adjustment (Rice, 1989).
Thus, essentially the same population structure is revealed from the analysis of random mating
and haploid populations.
The chi-square test of independence also showed that the ISI values for the "L-S" comparison in both locations did not depart significantly from zero confirming random mating
between the strains.
Inbreeding coefficients, co-ancestry coefficients, the expected inbreeding under random mating
and the deviation from random mating
were calculated using Evolutionary Algorithm for Mate Selection (Berg, 2012).
(1997) theorized that the large GD between the SS progenitors and its Cycle 0 was not inherent in the original population, but formed as a result of the continual random mating
necessary to maintain the cycles.
Expected probability of male philopatry under a hypothesis of random mating
is the probability that a male will pair with a female that returns to his natal colony or will pair with a female that immigrates to his natal colony.
socius females mated with heterospecific males according to random mating
frequencies, with the exception of cages A3, B1, B3, and C2.
Thus in the case of complete behavioral isolation, the ISI would be 1, and with no isolation (random mating
), the ISI would be 0.
The software package EVA (Berg, 2003) was used to obtain average inbreeding, average coancestry, expected inbreeding under random mating
, degree of nonrandom mating, and pedigree completeness indices.