recognition site


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recognition site

[‚rek·ig′nish·ən ‚sīt]
(cell and molecular biology)
The nucleotide sequence in duplex deoxyribonucleic acid (DNA) to which a restriction endonuclease binds initially and within which the endonuclease cuts the DNA.
References in periodicals archive ?
The number and modality of the recognition sites is very dependable in respect of molar relation between template molecule and functional monomer.
The DNA sequence for 5 amino acids (Asp-Asp-Asp-Asp-Lys) as a specific recognition site was inserted between PTH gene sequence and the fusion partner DNA sequences.
It happens because the chosen restriction enzyme (Bg/I) has two separated recognition sites (Fig.
The issues that remain to be determined include (a) where the proBNP processing occurs after secretion, (b) what protease works as a convertase for the secreted proBNP, (c) how the BNP convertase can access the recognition site in proBNP that is likely masked by glycosylation, and (d) whether CHF patients have any defect in the processing steps of secreted proBNP.
Point mutations located on the PARV4-O recognition site were retrieved in amplicons originating from samples positive with PARV4-N assay (Figure).
It is probable that the structure of different APNs keep a conserved conformation; however, the recognition site of Bt toxin could be a region susceptible to change.
Two restriction enzymes, MboI and BamHI, were run on all of the samples, even though the pattern recognized by MboI, a four base-pair recognition site, was included within the six base-pair recognition site of BamHI.
The company~s contract offer will be deemed to take into account all the findings of this recognition site, and include all accessories work and other ncessaires.
Third: Cpf1 cuts far away from the recognition site, meaning that even if the targeted gene becomes mutated at the cut site, it can likely still be re-cut, allowing multiple opportunities for correct editing to occur.
The method utilizes an oligonucleotide primer that contains a recognition site for a restriction enzyme such that digestion with the restriction enzyme generates a 5' overhang containing the locus of interest.