There was a recognition site
of Bc1I in CC genotype from the result of sequencing.
The number and modality of the recognition sites
is very dependable in respect of molar relation between template molecule and functional monomer.
The DNA sequence for 5 amino acids (Asp-Asp-Asp-Asp-Lys) as a specific recognition site
was inserted between PTH gene sequence and the fusion partner DNA sequences.
It happens because the chosen restriction enzyme (Bg/I) has two separated recognition sites
The issues that remain to be determined include (a) where the proBNP processing occurs after secretion, (b) what protease works as a convertase for the secreted proBNP, (c) how the BNP convertase can access the recognition site
in proBNP that is likely masked by glycosylation, and (d) whether CHF patients have any defect in the processing steps of secreted proBNP.
Point mutations located on the PARV4-O recognition site
were retrieved in amplicons originating from samples positive with PARV4-N assay (Figure).
It is probable that the structure of different APNs keep a conserved conformation; however, the recognition site
of Bt toxin could be a region susceptible to change.
Two restriction enzymes, MboI and BamHI, were run on all of the samples, even though the pattern recognized by MboI, a four base-pair recognition site
, was included within the six base-pair recognition site
The company~s contract offer will be deemed to take into account all the findings of this recognition site
, and include all accessories work and other ncessaires.
Third: Cpf1 cuts far away from the recognition site
, meaning that even if the targeted gene becomes mutated at the cut site, it can likely still be re-cut, allowing multiple opportunities for correct editing to occur.
The method utilizes an oligonucleotide primer that contains a recognition site
for a restriction enzyme such that digestion with the restriction enzyme generates a 5' overhang containing the locus of interest.