In order to elucidate this matter, we constructed a variant of Sema3C carrying point mutation (R745A) at the basic domain at the hypothetical furin recognition site
[sub.742][RNRR.sub.745], which would render Sem3C uncleavable at this particular location.
The LIG_CYCLIN_1 motifs in lined with amino acid residues amino acid residues RDLKV (323-327), which is a substrate recognition site
that act together with cyclin and increases phosphorylation of cyclin/cdk complexes.
The designed forward and reverse primers were as follows: forward primer introduced Hind III recognition site
underlined 5'-CAA TTA AA GCT TAT ATG CAT CAC CAT CAC CAT CAC ATG TCC TGC GGT AAC GCC AAG-3', and reverse primer introduced EcoRI recognition site
underlined 5'-CAT GGA ATT CTT ACT GCT TGC TGA AGT ATCC-3'.
Spike glycoprotein cleavage recognition site
analysis of infectious bronchitis virus.
One hypothesis proposed for the lac repressor system is that the lac repressor slides on one-dimensional DNA to find the recognition site
, and this hypothesis is supported by image analysis in living cells .
Since recognition sites
of MspI and NcoI enzymes were CCGG and CCATGG, both can be used in identification of G>A and C>T SNPs in calpastatin gene.
The number and modality of the recognition sites
is very dependable in respect of molar relation between template molecule and functional monomer.
It happens because the chosen restriction enzyme (Bg/I) has two separated recognition sites
The issues that remain to be determined include (a) where the proBNP processing occurs after secretion, (b) what protease works as a convertase for the secreted proBNP, (c) how the BNP convertase can access the recognition site
in proBNP that is likely masked by glycosylation, and (d) whether CHF patients have any defect in the processing steps of secreted proBNP.
FJ88355761), and more particularly those detectable on agarose gels but showing negative signal with PARV4-O TaqMan assay because of mismatches identified on the probe recognition site
The linker sequence contains a cleavage recognition site
for a disease specific protease such as a cancer, fungal, viral or parasitic protease.