As shown in figure 3, recombinant protein
was expressed and accumulated in the insoluble fraction.
Due to the existence of 6 His-tag at the C-terminus of proteins, Ni-NTA column was applied to purify recombinant protein
Also, identification of the produced recombinant protein
was specifically confirmed by SDS-PAGE and western blotting.
A conjugated with HiLyte[TM] Fluor 488 can be used for immunoglobulin detection in IHC and IF and for assay development.
The addition of Primorigen's Vitronectin XF and platform recombinant protein
production technology will play a huge role in Nucleus' strategy of offering traceable, reproducible and xeno-free cell culture ecosystems for the cell and gene therapy space.
Starting from a small supplier of proteins and enzymes for academic institutes and biotech companies, Creative BioMart has always been focusing on developing high quality protein products including recombinant protein
, GMP proteins, native proteins, assay kit, etc.
Based on the professional R&D ability and advanced technology platform, Amoytop Biotech has marketed three recombinant protein
drugs including rHuGM-CSF, rHuG-CSF and rHuIL-11.
expression in a Drosophila cell line: comparison with the baculovirus system.
In addition, Grifols recently began an engineering project to convert the 9,000 square foot expansion space within CMF into cell culture and purification suites for production of recombinant proteins
expressed in mammalian cells.
The company's new USD80m Consolidated Manufacturing Facility supports development and manufacturing services to third parties for recombinant protein
Expression of the Eg AgB8/2 recombinant protein
was carried out in [p.sup.ET32a(+)] vector in Escherichia coli BL21 (DE3).The cDNA was PCR amplified and cloned in the expression vector in frame with the vector fusion tag.
Expression of the recombinant protein
was induced with 0.5 mM IPTG at 37degC for 3 h and harvest bacteria liquid.