For example, reduced glutathione
(GSH) levels change from 71% of the reference (normal) value before therapy to 93% of reference value after 6 months of therapy, and SAM values change from 89.7% of reference value to 98% of reference value at 6 months.
However, curcumin treatment has restored the reduced glutathione
level in the brain tissues of PTZ-kindled mice.
It is our view that the present measurements of reduced glutathione
in the blood plasma are more indicative of the reduced glutathione
levels in the cells of interest in CFS than are the measurements reported in the literature, for these reasons.
Glutathione peroxidase activity (Gpx) (a), glutathione reductase activity (GR) (b), reduced glutathione
(GSH) (c), and glucose6-phosphate dehydrogenase activity (G6PDH) (d).
S-acetylglutathione is able to increase intracellular-SH groups as reported by Vogel et al., is more stable in blood plasma than GSH, and enters the cells directly, where it is converted to reduced glutathione
by the abundant cytoplasm thioesterases.
The assay system contained 2.2 ml of phosphate buffer (50 mM, pH 7.0), 0.1 ml EDTA (1.5 mM) 0.1 ml sodium azide (1 mm), 0.1 ml reduced glutathione
(1 mM), 0.1 ml NADPH (0.2 mM), 0.1 ml glutathione reductase (1 U/ml) and sutable amount of lysate to make the volume up to 2.8 ml.
(3) Nonstandard abbreviations: GSH, reduced glutathione
; LC/ESI-MS, liquid chromatography/electrospray ionization-mass spectrometry; Hb, hemoglobin; and metHb, methemoglobin.
The procedure to estimate the reduced glutathione
(GSH) level followed the method described by Ellman .
GSSH-Red regenerates reduced glutathione
(GSH) from oxidized glutathione, which has been formed by oxidation while CAT is responsible for the scavenging or detoxification of [H.sub.2][O.sub.2].
and total glutathione were higher in the epidermis than in the dermis by 513 and 471%.
The CEH-CDH method for determining serum cholesterol has several advantages over traditional methods: rapidity, simplicity, and no interference from various reductants, bilirubin, ascorbic acid, or reduced glutathione
. Moreover, because the amount of [beta]-NADH formed is equivalent to the amount of cholesterol, the concentration of cholesterol in serum can be estimated from a factor calculated from the measured molar absorptivity of [beta]-NADH calibrated by that in the Standard Reference Material.
Glutathione reductase (GSH-R, EC 188.8.131.52) is a flavoprotein that catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione