renaturation


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renaturation

[rē‚nach·ə′rā·shən]
(biochemistry)
The process of restoring denatured proteins to their original condition.
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Our hypothesis was that the refolding of BMP-2 under this mild condition (0.1 mM /0.1 mM GSH/GSSG) results in correct formation of intrachain disulfide bonds and therefore monomeric renaturation. Pooling with 0.5 mM 15 mM of GSH/GSSG pretreated BMP-2-which is oxidized rather than being in reduced state- resulted in improvement of protein dimerization and thus activity.
Marten et al., "Renaturation and purification of bone morphogenetic protein-2 produced as inclusion bodies in high-cell-density cultures of recombinant Escherichia coli," Journal of Biotechnology, vol.
Despite the optimization of the expression conditions for pearlin, it was mainly expressed in the form of inclusion bodies that needed to be solubilized in harsh conditions; renaturation and affinity purification were then used to obtain the physiologically active protein.
3 showed that the a-amylase activity was increased apparently when dextrin was carbon source on the condition of renaturation after SDS denaturation, and the a-amylase activity by dextrin with higher DE was higher than by dextrin with lower DE.
Brown coal surface-mining sites of the former German Democratic Republic undergo extensive renaturation, thus providing new biotopes for many plant and animal species, including ticks.
This will have to remain in the realm of prediction, as I cannot find any reference to the blackbody radiation spectrum of folding simple proteins as the temperature falls below the renaturation temperature.
Melting curve analysis was performed after amplification, starting with heating (95[degrees]C for 0 s) and cooling (50 [degrees]C for 30 s) steps for renaturation of the hybridization probes.
"In 1993 we came up with the revolutionary idea of refolding proteins by exposing them to a series of denaturation and renaturation steps, and the method resulted in a drastic improvement in the yield of correctly folded protein," said Hans Christian Thogersen, Borean's Chief Scientific Officer and Founder.
When equipped with the Peltier multi-cell holder, the Cary fluorescence spectrophotometer is ideal for life scientists conducting thermal denaturation and renaturation studies of DNA via fluorescence resonance energy transfer (FRET) and biochemists investigating the dynamics of proteins in solution via fluorescence polarization.
5,728,804-Use of cyclodextrins for protein renaturation. Issued May 17, 1998.
In recent years, high-throughput protein-refolding techniques have been developed for renaturation of inclusion bodies (20,21).