But with the ability to create intact, gene-altered reoviruses, scientists can now learn the precise functions of each reoviral gene by knocking out individual genes and observing what's lacking, structurally or functionally, in offspring viruses.
Ultimately, Joklik says, this should allow vaccine manufacturers to create reoviruses that can spur production of antireovirus antibodies in humans or animals without causing disease.
Joklik says he and his Duke colleagues will test their gene-insertion techniques later this month on reoviruses more dangerous than the innocuous strains they've tinkered with so far.
Similarly, new mammalian reoviruses, such as BYD1, JP, and BYL, were isolated from throat swab specimens of patients with severe acute respiratory syndrome (10).
The discrepancy initially observed between immunofluoresence and RT-PCR results may be attributed to the cross-reactivity of the Pan-Entero Blend reagent toward reoviruses, as mentioned in the manufacturer's description.
The sequences of the MRV2Tou05 S1 gene showed great diversity at the nucleotide and amino acid levels, compared with the S1 gene from mammalian reoviruses and SC-A strains (Tables 2, 3).
To establish the evolutionary relationship of MRV2Tou05 with the known mammalian reoviruses, we constructed phylogenetic trees on the basis of the nucleotide sequences of the S1 and S3 segments (Figure 3).
7) recently described a novel type 3 reovirus isolated from a child with meningitis and provided direct evidence that reoviruses can be neurovirulent in humans.
The S1 segment from reoviruses is highly diverse in size and sequence.
ARVs are not generally associated with encephalitic disease, in contrast to reoviruses that infect mice, baboons, and snakes (8,9).
Classification of Dutch and German avian reoviruses by sequencing the sigma C protein.