For the pgCAR dual-luciferase assay, NR1 as the CAR responsive element was cloned upstream of the firefly luciferase reporter gene
to pick up the ligands which regulate pgCAR causing altered reporter gene
If the serum contains neutralizing antibodies, these antibodies will prevent the drug from interfering with the TNF-induced induction of the reporter gene
, resulting in a luminescent signal (Fig.
If candidate A associates with candidate B in a yeast strain employed for screening, this two-hybrid has all the function of an active transcription factor that enhances transcription of the reporter gene
constitutively via binding to its cis-acting element, called the GAL4 binding site.
Using a cell-based assay system of STAT3 luciferase reporter gene
in HepG2 cells, we examined the PE, EtOAc, MeOH and [H.
After 24 h, plasmid estrogen response element (ERE)-LUC (1 [micro]g), a plasmid containing the ERE fused to the luciferase reporter gene
, [sup] or ERE-LUC together with the RACK-1 expressing pCMV-SPORT6 (MRC Geneserve, Mill Hill, London, UK) (1 [micro]g each) were transfected into the cells using Fugene Reagent (Roche, Burgess Hill, UK) in a ratio of 6 [micro]L/[micro]g DNA.
1987) reporter gene
for utilizing Arabidopsis as heterologous system to study resistance genes of roses in future.
Combinations of HTS assays measuring competitive ligand binding, reporter gene
activation, and enzyme inhibition can be used to characterize chemical potential for endocrine disruption.
Various recombinant parasites carrying a reporter gene
either as an episomal copy or after its integration in a defined locus, generally the rDNA locus against leishmaniasis are currently available (Table I).
With this type of direct analysis of transcript levels, the construction of cell lines with reporter genes
could be avoided altogether (Figure 3).
In other words, the reporter gene
is 'reporting' on the growth of the fungus," says Brown.
Fusion of the reporter gene
, GFP to each of the four Hc promoters has shown that all four strains have a functional M46 promoter.
The higher throughput of the six-channel instrument will allow small biotech and pharmaceutical companies a cost-effective approach to medium-throughput, Aequorin-based G protein-coupled receptor (GPCR) and Luciferase-based Reporter Gene
Screening," said Andy Boorn, president of MDS Analytical Technologies.