restriction endonuclease


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restriction endonuclease

[ri′strik·shən ¦en·dō′nü·klē‚ās]
(cell and molecular biology)
Any of the specific endonucleases that recognizes a short specific sequence within a deoxyribonucleic acid molecule and then catalyzes double-stranded cleavage of that molecule. Also known as endodeoxyribonuclease.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
References in periodicals archive ?
In prokaryotes, modification of DNA prevents cleavage by the cognate restriction endonucleases [2].
Five (Cfo I, Sau 96 I, Hpa II, Hin f I and Alu I) of the 12 restriction endonucleases cleaved the amplicons with more than one haplotype.
Digestion of amplified allele products with a restriction endonuclease can also provide very discrete typing results.
Polymorphism in mitochondrial DNA of humans as revealed by restriction endonuclease analysis.
Thirteen restriction endonucleases generated a total of 28 fragments; four of the restriction endonucleases (BamHI, EcoRI, PstI, SalI) made no cuts, while another restriction endonuclease (BglII) resulted in a single cut (Table 1).
Chromosomal digest by low-frequency cutting restriction endonuclease and pulse field gel electrophoresis revealed an identical pattern, suggesting a probable common origin.
Molecular epidemiology of Shigella infections: plasmid profiles, serotype correlation, and restriction endonuclease analysis.
For restriction endonuclease digestion, the blocks were washed three times with TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.6) prior to digestion.
The restriction endonuclease digest of the 511 bp COI PCR product resulted in 2 bands of 318 bp and 193 bp.

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