In prokaryotes, modification of DNA prevents cleavage by the cognate restriction endonucleases
For over ten years, the vast majority of molecular methods that analysed variation in DNA sequence relied on the special properties of the second major discovery, restriction endonucleases
Five (Cfo I, Sau 96 I, Hpa II, Hin f I and Alu I) of the 12 restriction endonucleases
cleaved the amplicons with more than one haplotype.
Digestion of amplified allele products with a restriction endonuclease
can also provide very discrete typing results.
Polymorphism in mitochondrial DNA of humans as revealed by restriction endonuclease
Thirteen restriction endonucleases
generated a total of 28 fragments; four of the restriction endonucleases
(BamHI, EcoRI, PstI, SalI) made no cuts, while another restriction endonuclease
(BglII) resulted in a single cut (Table 1).
Chromosomal digest by low-frequency cutting restriction endonuclease
and pulse field gel electrophoresis revealed an identical pattern, suggesting a probable common origin.
Molecular epidemiology of Shigella infections: plasmid profiles, serotype correlation, and restriction endonuclease
For restriction endonuclease
digestion, the blocks were washed three times with TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.6) prior to digestion.
The restriction endonuclease
digest of the 511 bp COI PCR product resulted in 2 bands of 318 bp and 193 bp.