restriction endonuclease


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restriction endonuclease

[ri′strik·shən ¦en·dō′nü·klē‚ās]
(cell and molecular biology)
Any of the specific endonucleases that recognizes a short specific sequence within a deoxyribonucleic acid molecule and then catalyzes double-stranded cleavage of that molecule. Also known as endodeoxyribonuclease.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
References in periodicals archive ?
In prokaryotes, modification of DNA prevents cleavage by the cognate restriction endonucleases [2].
For over ten years, the vast majority of molecular methods that analysed variation in DNA sequence relied on the special properties of the second major discovery, restriction endonucleases.
Five (Cfo I, Sau 96 I, Hpa II, Hin f I and Alu I) of the 12 restriction endonucleases cleaved the amplicons with more than one haplotype.
Digestion of amplified allele products with a restriction endonuclease can also provide very discrete typing results.
Polymorphism in mitochondrial DNA of humans as revealed by restriction endonuclease analysis.
Thirteen restriction endonucleases generated a total of 28 fragments; four of the restriction endonucleases (BamHI, EcoRI, PstI, SalI) made no cuts, while another restriction endonuclease (BglII) resulted in a single cut (Table 1).
Chromosomal digest by low-frequency cutting restriction endonuclease and pulse field gel electrophoresis revealed an identical pattern, suggesting a probable common origin.
Molecular epidemiology of Shigella infections: plasmid profiles, serotype correlation, and restriction endonuclease analysis.
For restriction endonuclease digestion, the blocks were washed three times with TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.6) prior to digestion.
The restriction endonuclease digest of the 511 bp COI PCR product resulted in 2 bands of 318 bp and 193 bp.

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