Although their existence had been hypothesised almost twenty years earlier, (23,24) the first restriction endonucleases
were not isolated until 1968.
Five (Cfo I, Sau 96 I, Hpa II, Hin f I and Alu I) of the 12 restriction endonucleases
cleaved the amplicons with more than one haplotype.
cleavage maps of animal mitochondrial DNAs.
Purified mtDNA was digested using 13 restriction endonucleases
, under conditions suggested by the supplier (New York Biolabs, Inc.
patent 9,458,439 which claims gene inactivation by use of chimeric restriction endonucleases
PacI, Pmel and SwaI were purchased from New England Biolabs (Ipswich, MA, USA).
Digestion spectrum of four selected restriction endonucleases
applied to 10 novel Chinese isolates and FV3 (the type species) to determine conflicts with the "standard spectrum" suggested by OIE.
The Stx-2A bacteriophages were highly similar in morphologic features, restriction endonuclease
profiles, chromosomal integration sites, and superinfection immunity (2,3) and showed <65% similarity to Stx phages from non-O104 strains.
Cloning kit (Inset/Aclone PCR product cloning kit), DNA extraction kit from Agarose gel kit, RT PCR kit and restriction endonuclease
were obtained from Fermentase (Switzerland).
A cladistic analysis of phenotypic associations with haplotypes inferred from restriction endonuclease
mapping and DNA sequence data.
org/content/vol57/ issue5, genomic DNA is first digested by a restriction endonuclease
(RsaI in this case) into fragments, one of which contains the entire target region between 2 closely spaced restriction sites.