restriction endonuclease analysis


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restriction endonuclease analysis

[ri‚strik·shən ‚en·dō′nü·klē‚ās ə‚nal·ə·səs]
(cell and molecular biology)
A technique in which deoxyribonucleic acid (DNA) fragments obtained from digestion with restriction enzymes are compared to construct a restriction map showing the position of specific sites along a sequence of DNA.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
References in periodicals archive ?
Johnson et al., "Comparison of seven techniques for typing international epidemic strains of Clostridium difficile: restriction endonuclease analysis, pulsed-field gel electrophoresis, PCR-ribotyping, multilocus sequence typing, multilocus variable-number tandem-repeat analysis, amplified fragment length polymorphism, and surface layer protein a gene sequence typing," Journal of Clinical Microbiology, vol.
Small fragment restriction endonuclease analysis in epidemiological mapping of group A streptococci.
Systematic relationships among waterfowl (Anatidae) inferred from restriction endonuclease analysis of mitochondrial DNA.
In conventional restriction endonuclease analysis, chromosomal or plasmid DNA is extracted from microbial specimens and then digested with endonucleases into small fragments.
Restriction endonuclease analysis of the PCR amplified hexon gene from representative four types of FAdV digested with BsiW1.
By using restriction endonuclease analysis of the 65-kDa heat shock protein gene (1), we found that the isolate showed a pattern compatible with Mycobacterium simiae.
Restriction endonuclease analysis of DNA from two isolates of Chlamydia psittaci obtained from human abortions.
Development of a rapid and efficient restriction endonuclease analysis (REA) typing system for Clostridium difficile and correlation with other typing systems.
The most widely used molecular typing methods include plasmid profiling, restriction endonuclease analysis of plasmid and genomic DNA, Southern hybridization analysis using specific DNA probes, and chromosomal DNA profiling using either pulsed-field gel electrophoresis (PFGE) or PCR-based methods (12,20).
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