restriction endonuclease analysis


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restriction endonuclease analysis

[ri‚strik·shən ‚en·dō′nü·klē‚ās ə‚nal·ə·səs]
(cell and molecular biology)
A technique in which deoxyribonucleic acid (DNA) fragments obtained from digestion with restriction enzymes are compared to construct a restriction map showing the position of specific sites along a sequence of DNA.
References in periodicals archive ?
Development of a rapid and efficient restriction endonuclease analysis typing system for Clostridium difficile and correlation with other typing systems," Journal of Clinical Microbiology, vol.
Comparison of seven techniques for typing international epidemic strains of Clostridium difficile: restriction endonuclease analysis, pulsed-field gel electrophoresis, PCR-ribotyping, multilocus sequence typing, multilocus variable-number tandem-repeat analysis, amplified fragment length polymorphism, and surface layer protein a gene sequence typing," Journal of Clinical Microbiology, vol.
Hae III restriction endonuclease analysis of PCR product revealed three different banding patterns consisting of 2-3 DNA fragments ranging from 0.
Small fragment restriction endonuclease analysis in epidemiological mapping of group A streptococci.
Systematic relationships among waterfowl (Anatidae) inferred from restriction endonuclease analysis of mitochondrial DNA.
Restriction endonuclease analysis has the advantage of being highly reproducible, very accurate in determining the relatedness of microbial strains, and well within the technical capabilities of experienced laboratory technologists.
Restriction endonuclease analysis of DNA from two isolates of Chlamydia psittaci obtained from human abortions.
Development of a rapid and efficient restriction endonuclease analysis (REA) typing system for Clostridium difficile and correlation with other typing systems.
The most widely used molecular typing methods include plasmid profiling, restriction endonuclease analysis of plasmid and genomic DNA, Southern hybridization analysis using specific DNA probes, and chromosomal DNA profiling using either pulsed-field gel electrophoresis (PFGE) or PCR-based methods (12,20).
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