restriction fragment


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restriction fragment

[ri′strik·shən ‚frag·mənt]
(cell and molecular biology)
Any of the individual polynucleotide sequences produced by digestion of deoxyribonucleic acid with a restriction endonuclease.
References in periodicals archive ?
Prevalence of the most frequent high risk-HPV types by PapilloCheck[R] microarray and restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP).
E., Restriction fragment length polymorphism analysis of PCR-amplified nifH sequences from wetland plant rhizosphere communities.
This restriction enzyme (Taq1) gave three different patterns with all isolated strains, isolated strains from pleural fluid and conjunctiva contained restriction fragment pattern (rfp1) and isolated strains from nasopharynx contained rfp1 and rfp2 (Figure 2).
Tabaqchali, "Rapid fingerprinting of Helicobacter pylori by polymerase chain reaction and restriction fragment length polymorphism analysis," Journal of Clinical Microbiology, vol.
Biodiversity degree: Microbial richness, the number of terminal restriction fragments (TRF)
In each case, DNA was double digested with the endonucleases and the resulting restriction fragments were ligated with the double-stranded adapters.
Simulate the action of the enzyme by separating each DNA molecule into its resulting restriction fragments (Figure 4).
From the restriction fragments of the amplified ITS- rDNA region of the 51 isolates were obtained 1 to 3 restriction enzyme fragments, which two profiles for Acc I enzyme, 3 for Hpa II, 4 for Eco RI, HinfI, Rsa I, Alu I, Taq I and Hha I (Table 1 and 2).
No sequence-derived information is extracted from restriction fragments, however, and no information is obtained on the order of fragment linkage.
Restriction fragments of PCR product of hsp65 and rpoB genes for reference strains of Mycobacterium and clinical samples Restriction fragment of hsp65 gene Restriction fragment sizes (bp) Reference strain/Clinical samples Hae III BstEII M.
A newer and rapidly developing way to characterize soil microbial communities is through the use of nucleic acid techniques such as terminal restriction fragment length polymorphism (TRFLP) analysis.
Additionally, individual samples representing the C/C, C/T or T/T genotypes analyzed by real-time PCR were also genotyped by DNA sequencing and restriction fragment length polymorphism techniques, proving the accuracy of the real-time PCR method.

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