Primers with introduced restriction sites
are also indicated.
Nevertheless, the available variability of the fragment lengths is naturally very restricted due to (i) the limited distance between two restriction sites
, and (ii) the usually very stringent size-selection step during library preparation.
Oligos Sequences Tm ([degrees]C) KpnLLexBpx_TEM GGTACCCTCTAWATATACACATGAGTATT 55 CAACATrrCCGTG KpnLpBR322 GGTACCACTCTTCCTnTTCAAT ATT ATT 50 pBR322_CS_seq AGCCTATGCCTACAGCATCCAG 62.1 In bold the restriction site
for Kpnl, in italic the lexA box consensus sequence, underlined the [bla.sub.TEM-1] gene.
Numbers in parenthesis indicate the number of species within each family containing the correct restriction site
. TABLE 2.
The "B" allele denotes absence of the Bsm1 restriction site
, while "b" allele indicates presence of the restriction site
Sequencing on a single lane of the flow cell yielded approximately 14 X [10.sup.6] paired-end reads, 97% of which were mapped to restriction sites
with the predicted fragment length and used for subsequent analysis (see Table 1 in the online Data Supplement).
This SNP introduced a new SmaI restriction site
into the Oka vaccine strain and provided the basis for developing a new PCR-RFLP technique for differentiation of wild-type VZV strains from the vaccine strain.
This study showed that the occurrence of a BsmI restriction site
(denoted b) in the intron between exons 8 and 9 of the gene is associated with enhanced lumbar spine BMD.
This study independently applies morphological characters and a key based on mitochondrial restriction site
variation to identify juvenile rockfishes collected in southern California during juvenile rockfish surveys.
The RNA transcript may be formed by linearization of the vector through cleavage at a unique restriction site
in a plasmid vector and then transcribing the linear molecule.
Table 1 Binary character matrix consisting of 19 restriction sites
. The presence of a restriction site
is denoted by a "1", while "0" denotes the absence of the site.
The lowland type has a restriction site
change that is not present in upland type.