Cyanine dye, DiO (3,3'-dioctadecyloxacarbocyanine perchlorate, Molecular Probes, D275), was used to trace the MLF axons from the rhombencephalon
In the preotic region, neural crest cells make the prepattern of trigeminal and facial nerves according to the segmental character of the rhombencephalon
(hindbrain), whereas the postotic crest cells have no rhombencephalic restriction but are limited in their migration by the anterior boundary of the trunk region, and thus make the prepattern of glossopharyngeal and vagus nerves (Shigetani et al., 1995; Ferguson and Graham, 2004).
The 3 primary vesicles are the forebrain (prosencephalon), midbrain (mesencephalon) and hindbrain (rhombencephalon
At HH stage 10, Mnb mRNA was detected at different levels of the closed neural tube: the prospective prosencephalon, mesencephalon, rhombencephalon
, and spinal cord (Figure 1i).
Otic vesicles, which form the structures of the inner ear, were seen as extensions of the rhombencephalon
. At 54 hours, the embryo began to twitch and colorless blood flowed through vessels.