Since in GeneXpert only rifampicin
resistance is reported so for the isoniazid resistance normal DST was performed.
1) and Rifampicin
resistance is nearly 20% among positive patient (Fig.
was measured by high-performance liquid chromatography using a Flexar[R] system (Perkin Elmer), after liquid-liquid extraction from plasma, following the procedure proposed by Zhou et al.
High prevalence of multidrug-resistant tuberculosis among patients with rifampicin
resistance using GeneXpert Mycobacterium tuberculosis/rifampicin in Ghana.
WHO estimates that in 2016 there were 600,000 new cases with resistance to rifampicin
- the most effective first-line drug-of which 490,000 had MDR-TB.
reduces plasma concentrations of moxifloxacin in patients with tuberculosis.
Since the patient was not receiving any other medications implicated in hemolysis, Rifampicin
was considered the likely culprit .
GeneXpert MTB/RIF detected an additional 2 patients with rifampicin
resistance in which the culture results were negative (Table 2).
Out of 40, GeneXpert positive patients, Rifampicin
resistance was detected in 2 patients (5%).
Detection of mycobacteria and Rifampicin
resistance was carried-out in the same setting.
Stock solution of the drugs was prepared based on the potency of the drug in sterile distilled water for streptomycin, isoniazid and ethambutol and in absolute methanol for rifampicin
tuberculosis with available TB drug susceptibility tests results for at least isoniazid and rifampicin
. In patients with multiple positive TB cultures, only the first positive culture was included.