where Ls is root length of the segment (m), DWr is total dry weight of root (segment of the root + remaining root) (g) and DWs is dry weight (g) of the root segment
Opaque dentine (with darker staining compared to adjacent dentine) was histologically seen in 8 roots: buccal root segment
(n=6), lingual root segment
(n=4) and circumferential in 1 specimen.
X-ray computed tomography (CT), which is widely used in the medical sciences, may be applied to acquire cross-sectional image slices of a root segment
(Tracy et al., 2011).
Inward fragmentation of the oral root segment
opens the view to the apical alveolar space to obtain access to the anatomically critical apical root segment
Each intersection was searched for the nearest root segment
. The optical pointer in the eyepiece of the microscope was placed on the root segment
, and the cell directly below the pointer was examined at 100X magnification.
([paragraph]) Percent infection sites associated with GUS staining in a hairy root segment
not expressing GUS activity.
(1) what kind of access should there be to the root segment
through its (unique) key and
The root segment
with the highest score (poorest removal of filling materials) was applied for the statistical analyses in each case.
Once all the roots were scanned, a small quantity of 1-cm root segments
from each plant were cleared with 10% KOH and stained with trypan blue (Phillips and Hayman, 1970).
The root segments
were transferred to a clean beaker and soaked in water.
The root segments
were randomly selected and mounted on a glass slide in groups of 10.
To quantify mycorrhizal colonization, root segments
totaling >10 cm from each individual were cleared in 10% KOH, boiled in an ink-vinegar solution to stain fungal structures (Vierheilig et al, 1998), and mounted using PVLG (polyvinyl alcohol-lactic acid-glycerol).