Safranine

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safranine

[′saf·rə‚nēn]
(organic chemistry)
Any of a group of phenazine-based dyes; some are used as biological stains.

Safranine

 

any of a class of azine dyes. The simplest safra-nine is phenosafranine, with the structural formula

where R1 = NH2 and R2 = R3 = R4 = H. Phenosafranine is a red dye formed in small quantities upon the oxidation of a mixture of aniline and p-phenylenediamine. It has very few uses.

Tolusafranine, referred to commercially as safranine (R1 = NH2; R2 = R4 = CH3; R3 = H), is a bright red basic dye. It is formed upon the oxidation of a mixture of aniline, o-toluidine, and p-tolylenediamine. Tolusafranine was used until the 1940’s as a dye for leather, paper, and textiles. Today, it has been almost completely replaced by dyes that are less expensive and more colorfast.

Pinakryptol (R1 = R2 = R4 = H; R3 = NH2) is a green dye obtained by the action on o-aminodiphenylamine of, first, picryl chloride and second, zinc with hydrochloric acid. It is used as a desensitizer in photography for lowering the sensitivity of photographic plates. All safranines are toxic.

M. A. CHEKALIN

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These results show that the PHA hydrogel rapidly swollen, and the PHA hydrogels containing EGDMA swollen about 10-14 times more than those PHA hydrogels containing NNMBA Moreover, while Safranin T swelled PHA-EGDMA hydrogel 1.33-1.43 times more than other dyes, it swelled PHA-NNMBA hydrogel 1.02-1.13 times more than the others.
Gupta, "Removal of Safranin dye from aqueous solution using magnetic mesoporous clay: Optimization study," Journal of Molecular Liquids, vol.
(a) Representative histological analysis (Safranin O staining) of the knee joint in MIA-induced OA.
In addition, a uniform distribution of safranin O staining was observed, and the tide line appeared intact.
The UF range was estimated from the tidemark to the furthest recognizable chondrocyte within the ligament, as described previously,11 and this area was stained in red through Safranin O staining (Fig.1b).
Leaf sections were then placed on the slide and added one to two drops of safranin, and the extra safranin was removed by pouring few drops of 96% ethanol.
[DELTA][[PSI].sub.m] was estimated by a spectrofluorometric assay using Safranin O [20].
The slide was washed with water and a counter staining agent, (safranin solution) was poured to the slide and was left again for a full minute.
After the cells were fixed in 95% ethanol (30 min), they were successively stained with 0.02% aqueous Fast Green for 5 min (Sigma) and 0.1% safranin O for 10 min (Sigma), immediately washed with tap water, and dried naturally at room temperature.
Subsequently, 5-[micro]m-thick sections from the mid-sagittal plane of the PPT complex were stained with Safranin O to examine the BTJ proteoglycan profile and with hematoxylin & eosin (H&E) to examine the general morphology; the latter analysis included an assessment of the tendon collagen fibers under a polarized microscope (Nikon Eclipse 50i, Nikon Inc., Japan).
Slices were mounted on glass slides and stained with Safranin O (Sigma-Aldrich[R]) and fast green (Sigma[R]).
Sections of 15-20 p were taken on sledge microtome and double stained with Safranin - Fast green.