Safranine

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safranine

[′saf·rə‚nēn]
(organic chemistry)
Any of a group of phenazine-based dyes; some are used as biological stains.

Safranine

 

any of a class of azine dyes. The simplest safra-nine is phenosafranine, with the structural formula

where R1 = NH2 and R2 = R3 = R4 = H. Phenosafranine is a red dye formed in small quantities upon the oxidation of a mixture of aniline and p-phenylenediamine. It has very few uses.

Tolusafranine, referred to commercially as safranine (R1 = NH2; R2 = R4 = CH3; R3 = H), is a bright red basic dye. It is formed upon the oxidation of a mixture of aniline, o-toluidine, and p-tolylenediamine. Tolusafranine was used until the 1940’s as a dye for leather, paper, and textiles. Today, it has been almost completely replaced by dyes that are less expensive and more colorfast.

Pinakryptol (R1 = R2 = R4 = H; R3 = NH2) is a green dye obtained by the action on o-aminodiphenylamine of, first, picryl chloride and second, zinc with hydrochloric acid. It is used as a desensitizer in photography for lowering the sensitivity of photographic plates. All safranines are toxic.

M. A. CHEKALIN

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The Safranin O staining revealed changes in the proteoglycan profile in the injury groups.
The thick red masses observed in the pathway of the white mango scale SB and neighboring plant cells after staining with safranin were presumed to be phenolic deposits, since Zamora-Magdaleno et al.
These fragments were stained with 1% safranin in 50% ethanol (Berlyn & Miksche 1976), and mounted in Kaiser's jelly glycerin for frontal view (FV) observations.
Tissue samples that previously (24h before) had been stored in 10% phosphate-buffered formalin, decalcificated by solution containing 10% formalin and 10% formic acid, and then were embedded in paraffin and 5-611[micro]m sections were cut using a rotary microtome and stained with specific cartilage staining technique of safranin 0.
For staining, safranin was used for lignified tissues (sclerenchyma, xylem vessels) and fast green for subrinized and parenchymatous tissues.
1); fiber portions of 1 cm length were macerated in 5% sodium hydroxide solution, boiled for 30 minutes, bleached with 5% chloral hydrate, and stained in 1% safranin (Msahli et al.
When sections stained by hematoxylin/safranin O/fast green method, the cationic dye safranin O stained the produced sulfated glycosaminoglycan of matrix by the chondrons intensely red (Figure 7B).
Safranin solut yukunun artmasi ile tikaclar olusur, sitotoksin ve bakterilerle de birleserek safra yollarina zarar verir.
5] has reported the electrochemical efficiency for releasing Safranin molecule by CP composite film using open circuit condition and also with a couple of reduction-oxidation cycles separately.
Endospore staining was carried out by heating the air dried bacterial smear with malachite green for 5 min, followed by rinsing with water and counterstaining with safranin for 30s.
The samples processed by the Ritchie method were stained by the modified Ziehl Neelsen procedure and modified safranin technique for the identification of oocysts of coccidians (7).