immunoassay

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Immunoassay

An assay that quantifies antigen or antibody by immunochemical means. The antigen can be a relatively simple substance such as a drug, or a complex one such as a protein or a virus. See Antibody, Antigen

The reactants are first mixed so that a varying quantity of one (A) is added to a constant amount of the other (B). The formation of an immune (antigen-antibody) complex is measured as a function of the varied reactant (A). The result is represented by a “standard curve” for reactant A. An unknown sample is tested by adding it to reactant B. The extent of the measured change is referred to the standard curve, and thereby is obtained the amount of reactant A which produces a comparable change. The amount is represented as the content of reactant A in the unknown sample. See Immunofluorescence, Immunology, Radioimmunoassay

McGraw-Hill Concise Encyclopedia of Bioscience. © 2002 by The McGraw-Hill Companies, Inc.

immunoassay

[‚im·yə·nō′a‚sāy]
(immunology)
A laboratory detection method that uses antibodies to react with specific substances.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
References in periodicals archive ?
To prepare the assay calibrator for total 25(OH)D sandwich immunoassay on an automated immunoassay platform, we dissolved crystalline 25(OH)[D.sub.3] in 99.9% ethanol to make a stock solution at 10 ig/mL, and we prepared calibrators containing 200, 100, 50, 25, 12.5, 6.25, and 3.13 ng/mL 25(OH)[D.sub.3] by further diluting the stock solution with horse serum.
Sandwich immunoassay as a highthroughput screening method for cross-coupling reactions.
Plasma concentrations of sGPVI were determined by use of a bead-based sandwich immunoassay and mAb 8E9 reactive with human GPVI as capture antibody.
Clinical assays for cardiac troponins are sandwich immunoassays directed to stable portions of cardiac cTnI and cTnT.
Multiplexed cytokine sandwich immunoassays are capable of measuring dozens of cytokines at the same time.
BNP immunoreactivity was analyzed using 2 sandwich immunoassays: one utilizing monoclonal antibody (mAb) KY-BNP-II (epitope 14-21) as capture with mAb 50E1 (epitope 26-32) for detection and a single-epitope sandwich BNP (SES-BNP) immunoassay specific to the epitope 11-17.
Multiplexed cytokine sandwich immunoassays are not capable of measuring many cytokines at the same time.
The stepwise miRNA assay protocol is much slower than routine simultaneous incubation sandwich immunoassays (e.g., sandwich immunoassays performed on the Advia Centaur[R] analyzer used in this work only require 7.5-min incubations).
In this study, commercial anticTnI MAbs specific to different epitopes were used as capture antibodies in sandwich immunoassays with an anti-troponin C MAb used as a tracer.
* PRL is commonly measured with sandwich immunoassays that use chemiluminescent or electrochemiluminescent techniques.
Sandwich immunoassays have proved to be an excellent method for accurately quantifying biomarker candidates.