with 1,10- phenanthroline and some biologicaly important amino acids like glycine, L-Valine and Sarcosine
(N-methyl glycine) as lignds have great socio economic importance and unique significance in the field of biochemistry.
We present measurements in plasma of prostate cancer patients characterised by PSA, tumour stage and Gleason score using GC/MS for sarcosine
methodology (9,10) to elaborate further on the suitability of sarcosine
as a marker for prostate cancer.
(N-methylglycine) is a naturally occurring GlyTl inhibitor that has been used in early clinical trials in Taiwan.
Later, a 2 per cent N-lauroyl sarcosine
was added to this reaction mixture and incubated for one hour.
Arun Sreekumar, the study not only proves that sarcosine
is a marker of cancer aggressiveness, but also that it is highly associated with tumour development as well.
The latest study links a substance called sarcosine
- produced by the body - to the tiger strain.
They also found that this molecule, known as sarcosine
, may be associated with prostate cancer invasiveness and aggressiveness.
Urine creatinine concentrations were measured using Creatinine Plus Assay, which is a coupled enzymatic reaction using creatininase, creatinase, and sarcosine
oxidase (Roche Diagnostic, Indianapolis, Ind).
Lam-otrigine has received the most attention; other agents include glycine, D-alanine, and sarcosine
5% sodium lauryl sarcosine
[Sarcosyl, Kodak International Biotechno-logies, New Haven, CT, USA], 1 mg/mL lysozyme [Life Technology, Sigma-Aldrich, Steinheim, Germany], and 7 [micro]g/mL lysostaphin [Sigma]), then incubated overnight at 55[degrees]C in 4 mL Lysis 2 buffer (1% sodium lauryl sarcosine
This method allowed determination of concentrations of 20 common amino acids, as well as phosphoserine (P-Ser), taurine (Tau), urea, citrulline (Cit), sarcosine
(Sar), [alpha]-aminoadipic acid ([alpha]-AAA), [beta]-alanine ([beta]-Ala), [beta]-aminoisobutyric acid ([beta]-AiBA), [gamma]-aminoisobutyric acid (GABA), ethanolamine (EtON[H.
After that, the homogenate was washed with 50 [micro]l 3% sarcosine
Tris-HCl and 1.