The assembly shall consist of two independent inverted microscopes, the first one in the arrangements for observing in the broad field with epi-fluorescence and TIRF excitation and second confocal arrangement with the rapid scanning spot. The system must contain superresolucnE[degrees] module based on stochastic localization of different fluorochromes (SMLM) and at least one other super-module and resolution based on stimulated emission depletion (STED) and / or on the basis of structured excitation (SIM).
An inverted fluorescence microscope in a research confocal arrangement with fast scanning spot;
The STED CW system is based on Leica's STED technology, which scales down the scanning spot
to sub-diffraction size by switching off fluorescence in the periphery of the excited area.
Like the scanning spot
triangulation systems, the line setup delivers a height profile along the projected structured light line (Iakovou, 2009).
The DLS of the UK firm is matchless in the midst of laser vision sensors intended for welding since it applies the scanning spot
method in place of the hugely used technique of laser stripe.
where diamtr is the number of pixels which form the curve segment between [c.sub.1] and one of the intersections at which the curve and the edges cross each other (i.e., diamtr is half of the length of the curve segment); u[1 j] and l[1 j] are pixel values of they th scanning spots when a scanning is conducted from [c.sub.1] to each of the upper intersection and the lower one along the curve.
where [lenght.sub.m] is the number of the shifts in [R.sub.m], diam r[k] is the number of pixels which form the curve segment between [c.sub.k] and one of the intersections at which the kth scanning curve and the edges cross each other, and then, w[k, j], and l[k, j] are pixel values of the jth scanning spots when the kth scanning is conducted from [c.sub.k] to each of the upper intersection and the lower one along the k th scanning curve, respectively.