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A GC-MS analysis of trimethylsilyl derivatives of this fraction indicated the presence of phenolic compounds such as gallic acid and the alkaloid securinine.
Previously, alkaloids such as viroallosecurinine and securinine (Mensah et al.
Securinine was purchased from Abeam Biochemicals, U.
After literature search of the possible biological activities of the identified compounds (1-29), we suspected that securinine and gallic acid could contribute to the cytotoxicity of the extract of M.
discoidea extracts, chemical analysis by GC-MS and cell proliferation assay showed that securinine and gallic acid could be the cytotoxic compounds in the M.

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