Tubules and interstitial tissue volume evaluation: The point counting method was applied to estimate the volume density and volume of the seminiferous tubules
and interstitial tissue at the final magnification of 280x:
Alkaline phosphatase (ALP) is found in interstitial cells, seminiferous tubule
and basement membrane, playing a key role in transfer of materials from sertoli cells to germinal cells and growth of the germinal epithelium20.
Spermatogenesis was assessed by a method which depends upon scoring 'cross sectional' profiles of seminiferous tubules
according to Johnson, using Johnson's score.
For morphometric analysis, 15 seminiferous tubules
from each goat were photographed under microscopy with a 10x objective, after which the photomicrographs were used to measure the tubular diameter by means of ImagLab 3000 software.
Percentages of seminiferous tubules
containing multinucleated giant cells
Testicle weight, length, breadth, seminiferous tubule
diameter and circumference in vaccinated (Group T) and control (Group C) ram lambs at week 30 after primary immunization (age for 38 weeks) Traits Group Testicle Testicle weight length (g) (cm) T 190.97 [+ or -] 28.09 6.52 [+ or -] 0.12 C 207.70 [+ or -] 28.23 6.69 [+ or -] 0.21 Traits Group Testicle Seminiferous breadth tubule diameter (cm) ([micro]m) T 4.74 [+ or -] 0.15 205.05 [+ or -] 14.21 C 4.78 [+ or -] 0.54 206.89 [+ or -] 15.62 Traits Group Seminiferous tubule
circumference ([micro]m) T 672.82 [+ or -] 41.36 C 677.07 [+ or -] 44.05 * There was no significant difference between vaccinated (Group T) and control (Group C) for all traits in this table.
The European Guidelines on male infertility recommend Johnsen's scoring for the assessment of the stage of spermatogenesis in the seminiferous tubules
The tubular compartments were comprised of the seminiferous tubules
, straight seminiferous tubules
and rete testis, without any distinct boundary tissue between them.
The large nuclei of spermatocytes in the pachytene stage were located in the middle of the seminiferous epithelium, while the nuclei of Sertoli cells, type A spermatogonia, and intermediate spermatogonium were closer to the basal lamina of the seminiferous tubule
Sections were examined for Johnson scoring, diameter of seminiferous tubules
, thickness of germinal epithelium.
Caption: Figure 4: Microphotographs of cross sections of the seminiferous tubules
in the control, ischemia-reperfusion, and treatment groups (hematoxylin and eosin staining, original magnification x200).
Previous studies have shown that the germinative epithelium is significantly reduced and that seminiferous tubule
diameter increases with age in sufferers of prune belly syndrome [5-8].