The remaining aqueous extract was further fractionated into ethyl acetate fraction by using 500 ml ethyl acetate for three times in a separatory funnel
to obtain the solid residue (4.5 g).
The contents were poured through a funnel into a 100 mL separatory funnel
, along with 3 mL of 0.1 M NaCl in Milli-Q water.
Ethyl acetate was added to the water layer obtained after the partition with hexane with 1: 1 ratio (v/v) in the separatory funnel
. The funnel was shaken for 2 minutes and left at room temperature for 2 hours to settle down.
After 30 min reaction to attain constant state, 200 mL solution is taken out from phenanthrene reaction system, followed by introduction into 400 mL separatory funnel
. 6g NaCl is added into the filtered solution.
After slowly inverting the separatory funnel
, two distinct layers were formed.
The obtained suspension was filtered by collecting the liquid into a separatory funnel
to separate the phases, and then the lipid extract was dried as reported earlier.
Details of the formulations used for unfunctionalized silica nanocomposites Formulation Factors F1 F2 [n.sub.HMDA]/ 3.0 3.3 [n.sub.ADCI] (a) VolumeTEOS (ml) 10 2.1 Reaction Time (sec) 90 120 Components added from HMDA & TEOS in water AdCl in toluene separatory funnel
to (aqueous phase) (organic phase) initiate reaction (a) The symbol n represents mole.
Flanagan notes that the way to check a phase separation in the laboratory is to dump the contents of a flask into a separatory funnel
, shake the funnel and wait for the phases to separate.
A total of 1.0 ml (density = 1.04 g/ml) of both plant essential oils were transferred to a 125 ml separatory funnel
and dissolved in 50 ml of hexane.
This causes the n-hexane to locate itself in the upper portion of the separatory funnel
upon standing after shaking.
The water sample was transferred from the sample bottle to a 1000 mL separatory funnel
. Thirty mL of methylene chloride were added to the sample bottle, which was sealed and shaken for 30 seconds to rinse the inner surface.
The upper clear green ether phase was carefully decanted into a separatory funnel
and transferred to a pre-weighed round-bottom flask.