Overall sequence error
was the number trials in a block (five trials) in which the sequence order was wrong.
In this section, the expected homozygous (parents carried same homozygous SNP) and heterozygous (parents carried different homozygous SNP) would be used to estimate the sequence error
rate and MAR respectively.
Both sequence error
(packets arriving out of order) and misordered packets (packets arriving out of order, independent of frame loss) should be identified.
"We had to look for sequence errors
in patients that could result in functional changes or loss of function for the encoded protein," said Wang, a co-corresponding author.
Amplification was performed using a mixture of high fidelity (Pfu) polymerase to minimize the incorporation of sequence errors
and standard Taq polymerase to facilitate cloning by the addition of an A tail to the products.
prowazekii based on SNPs between closely related strains may be complicated by PCR and sequence errors
. Conversely, our method confers more confidence in the validation of the mutations because we differentiated all strains except for 2 flying squirrel strains by insertion and deletion mutations, which are rarely generated by PCR or sequence errors
Since this type of analysis required the availability of corresponding intervals across trials, only reproductions without sequence errors
could be included.
Recently, 2 methods, Safe-Sequencing System (Safe-SeqS) and duplex sequencing, were reported to enable more sensitive and accurate rare variant detection by stringently eliminating sequence errors
In mental flexibility and alternating attention evaluations, assessed by Trail Making Test (TMT)-Part B, the SG performance showed a statistically significant difference compared to the CG on all evaluated items, i.e., the group composed of subjects with ADHD required more time to perform the test, has committed more alternating and sequence errors
, as shown in Table 3.
To test whether these last two discrepancies were either sequence errors
in the GenBank p53 sequence or mutations specific to HeLa cells, we amplified and isolated the p53 gene from normal human genomic DNA from five to six people, using the same protocol as for single cells.