Isolation of Antibiotic Resistant Bacterial Strains Ampicillin resistant bacteria were isolated from fruits (apple, peach, grapes and persimmon) and from vegetables (lettuce, carrots, tomatoes and peppers) on ampicillin (150ug/ml and 250ug/ml) supplemented L- agar plates by serial dilution
5 dilutions of the actual semiquantitative titer when measured by serial dilution
The assay requires preparation of the specimen into serial dilutions
to detect the point at which the antibody is no longer detected.
Application of this approach requires one to generate a standard curve for your reaction, using some form of quantified target with a known value for your template to create a serial dilution
and prepare a standard curve for reference.
1 ml of the previously prepared serial dilution
Diagnosis of allergy by serial dilution
skin end-point titration.
Sensitivity of culture methods and PCR was determined by percent recovery and by using serial dilutions
11 Approximately 50 ml of the juice samples were blended with 450 ml of butter field's phosphate buffer for 2 minutes and a serial dilution
of (10-1 to 10-4) were made.
The sensitivity of the quantitative PCR assay was assessed using a serial dilution
automated pipetting in key fundamental applications such as plate replication, serial dilution
and reagent additions, while also being able to address more challenging applications like MALDI spotting, nucleic acid extraction, ELISA and solid phase extraction," said Rick Luedke, product manager for Liquid Handling Systems at Hamilton.
The starting reagent concentration, of course, will not affect the systematic serial dilution
process but it should be known and understood.
The conventional serial dilution
ELISA has been modified to a recombinant antigen based single serum dilution ELISA to reduce the reagent costs and increase the number of the samples to be screened as described earlier for canine leptospirosis (6).