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Lastly, the authors state that our article advocated interpreting a negative sickling test as the patient being negative for the disease, with no further testing required.
When the sickling test is used together with the haemoglobin electrophoresis method, USh 9 565 912 would be incurred in eastern Uganda under scenario B1 and USh 7 147 912 in scenario B2 in year 1, thereby saving USh 2 418 000.
One of the consideration to be taken into account in making a diagnosis of HbD is to rule out Hb S as both share same band positions when cellulose acetate electrophoresis at alkaline pH is done, so sickling test becomes mandatory, but with HPLC both the bands can be differentiated.