The protein pellet obtained after each step was dissolved in 20 mL of 50 mM sodium acetate
buffer (pH 5) and was utilized to examine the enzyme activity.
The research paper - Integration of Anhydrous Sodium Acetate
(ASAc) into Concrete Pavement for Protection against Harmful Impact of Deicing Salt - is available now through the journal The Journal of The Minerals, Metals & Materials Society.
The enzyme activity assay mixture was prepared by mixing 50 uL of recombinant cellulase with 1% sodium carboxymethyl cellulose in 50 mM sodium acetate
buffer (pH 4.8) containing 2.5 mM cobalt.
They are indicated for the hydration of calves, are supposedly alkalizing, having sodium bicarbonate or sodium acetate
, and contain glucose or lactose as energy source.
Aside from biosilica xerogel, 1.663 g of sodium acetate
can also be derived from synthesis through evaporation of the aqueous solution (Figure 1).
We further tested other run buffers such as sodium phosphate, potassium phosphate, sodium acetate
, and ammonium acetate using different concentration combinations (between 20 and 100 mmol/L) and pH (between 2.5 and 4.5).
Effects of pH on activity were determined from pH 3-11 at standard conditions using 25 mM of each buffer, sodium citrate (pH 3), sodium acetate
(pH 4-5), sodium phosphate (pH 6-7), Tris-HCl (pH 8-9) and sodium carbonate (pH 10-11).
Anaerobic Digestion of Microalgal Biomass and Sodium Acetate
. Results on utilization of a UASB reactor (Supplemental Figure 1) to digest a mixed feedstock of microalgae and sodium acetate
are described in a recently published paper by two of this paper's authors  and this research is specifically aimed at results from analysis of microbial community that lead to the process of anaerobic digestion.
Dialysis: After precipitation the excess salts in the enzyme solution was removed by the dialysis using 1mM sodium acetate
buffer at a pH of 5.5 by using 12-14 kDa cut off membrane (Cellu Sep).
Group 1 (control group) was given sodium acetate
10mg/kg body weight in the intraperitoneal injections whereas Group 2 (lead group) and Group 3 were given lead acetate 10mg/kg body weight by intraperitoneal injection, for six weeks.
Pellet was suspended in 50 mM sodium acetate
buffer (pH 5.2) and stored at 4C.
Five isonitrogenous diets were formulated to contain different organic acid salts, which included the control (no added organic acid salt), sodium citrate, sodium acetate
, sodium propionate, or sodium butyrate added at 2%.